Clean Catch Urine Sample

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For what type of testing is a clean catch midstream urine specimen needed? 

Analyzing a clean catch urine sample helps in the diagnosis of infections of the urinary tract. Common tests that require clean catch samples include: 

  • Urinalysis 
  • Urine culture
  • Sensitivity testing
  • Microscopic examinations
  • Urine cytology
  • Pregnancy tests

How to do a clean catch urine sample 

Supplies needed

  • Gloves
  • Collection container
  • Label with client identifiers
  • Urinary-prep wipe 
  • For catheter-obtained samples:
    • Luer-lock syringe
    • Prep pad

Steps for client-obtained samples

Clean catch urine samples are usually collected by the clients themselves, but some may require assistance. 

  1. Wipe the external genitalia with a cleansing wipe. Instruct male clients to keep the foreskin retracted; in females, separate the labia and wipe front to back, left, right, and middle with separate towelettes.
  2. Start urinating into the toilet.
  3. Stop urinating and hold the collection cup in place.
  4. Begin urinating again into the collection cup.
  5. Label the specimen with date, time, and client identifiers.

Steps for catheter-obtained samples 

  1. Perform hand hygiene.
  2. Check tubing for urine. If tubing is empty, clamp ~ 3 inches below the sample port.
  3. Clean sample port with prep pad.*
  4. Attach the Luer-lock syringe to the sample port and withdraw 10–30 ml of urine.
  5. Detach syringe and remove tube clamp.
  6. Open sterile container, transfer urine, avoiding touching the syringe to the container. 
  7. Close the container, label the specimen.

*If the sample port is not present (step 3), clean the catheter valve with a prep wipe and transfer urine into a sterile container, avoiding touching the valve to the container. Close the container, label the specimen.


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